|
KMID : 0359320010410030333
|
|
Korean Journal of Veterinary Research
2001 Volume.41 No. 3 p.333 ~ p.342
|
|
|
Sequence analysis of the hypervariable region in VP2 gene of infectious bursal disease vaccine strains
|
|
권혁무/Kwon, Hyuk Moo
박유진/김수정/Park, Yoo Jin/Kim, Soo Joung
|
|
|
Abstract
|
|
|
|
To detect the genetic variations among infectious bursal disease (IBD) vaccine strains, the hypervariable region of VP2 gene of seven IBDV vaccine strains were amplified using reverse transcriptase/polymerase chain reation (RT/PCR). Ampllified PCR products of IBDV were cloned, sequenced, and compared with published sequences for IBDV. Vaccine strains (JOONG,HAN,B7,IB,BU2,G2,CIL) used in Korean and Korean field isolates (SH/92, K1, 310) had 81%(310 and HAN)~98%(SH/92 and CIL) amino acid sequence similarity. Vaccine strains had 80%(HAN and IB)~99%(JOONG and BU@) amino acid sequence similartiy. Intermediate plus vaccine strain, CIL was not substituted at positions 279(D→N) and 284(A→T), and conserved in serine-rich heptapeptide. At the two hydrophilic region, JOONG, IB and Bu2 strains had identical amino acid sequence comparing with STC strain. By phylogenetic analysis, JOONG and DAE strains were categorized in same group with BU2. The CIL and STC strains closely related but seperated from G2, HAN, B7 and IB strains.
|
|
|
KEYWORD
|
|
|
Infectious bursal disease virus(IBDV), hypervariable region, VP2 gene, RT/PCR, sequence
|
|
|
FullTexts / Linksout information
|
|
|
|
|
|
Listed journal information
|
|
|
|